Reticulofibrillar staining and detection of immunophenotypic markers of malignancy are helpful in the diagnosis of HCC.[1] Some researchers found that Glypican-3 mRNA and protein levels were increased in 74.8% of HCC patients, but not expressed or expressed at low levels in benign liver disease patients and normal subjects.[8,9] This suggests that the abnormal expression of Glypican-3 is closely related to the development and malignancy of HCC. The gene discussed is GPC3; the disease is hepatocellular carcinoma.