The core components of semen strychni used in the treatment of glioma included stigmasterol, (S)-stylopine, isobrucine, icaride A, and isostrychnine N-oxide (I), which may operate on core targets such as ADRB1, ADRB2, CHRM3, ADRA1A, and ESR1. The gene discussed is ESR1; the disease is central nervous system cancer.