To validate this concept, we engineered NeoViron, a multifunctional OAV platform incorporating two synergistic components: (1) tandem minigenes encoding validated neoantigens from murine tumor models, and (2) murine Fms-like tyrosine kinase 3 ligand (Flt3L), a dendritic cell (DC) growth factor known to expand intratumoral conventional type 1 dendritic cells (cDC1s) critical for antigen cross-presentation.16 NeoViron is designed to synergize with four complementary antitumor mechanisms. The gene discussed is FLT3LG; the disease is neoplasm.