Subsequently, single-sample gene set enrichment analysis (ssGSEA) was conducted using the deconvoluted melanoma cell profiles from MM samples obtained from the TCGA-SKCM dataset to explore signaling pathways associated with KPC1. These analyses revealed that KPC1 levels in MM cells were negatively correlated with the ssGSEA hallmark EMT pathway score (Fig. 2E, F). Here, RNF123 is linked to melanoma.