However, the regulation of EEC-MHCII expression in vivo, and its contribution to EoE, is unknown.<h4>Objective</h4>The objective of this study was to determine the regulation and role of EEC-intrinsic MHCII expression in EoE.<h4>Methods</h4>We examined the expression of HLA II-pathway transcripts in human EECs using single cell RNA-seq datasets and primary human tissues and mouse systems to interrogate the contribution of IFNγ to EEC-MHCII expression. Here, IFNG is linked to eosinophilic esophagitis.