In summary, while IFNγ and IL‐2 production from bulk T cells, as measured by Fluorospot, was maintained over time post‐infection in the complete cohort (Figure 3), a more detailed examination of S‐specific CD4+ T cells by ICS revealed subset and polyfunctional (3–4 cytokine) responses that declined over time (Figure 5c). This evidence concerns the gene CD4 and infection.