TP53 and cancer: The tumor suppressor protein p53 regulates the cell cycleand prevents cancer cell proliferation, and chalcones can upregulatep53 by disrupting p53-MDM2 interactions, modulating proteins likeSp1, CRM1, and HSP40, and restoring p53 pathways., Chalcones also act as antimitotic agents by binding to tubulin,inhibiting its polymerization and disrupting mitotic spindle assembly,leading to cell cycle arrest in the G2/M phase and apoptosis.