Regarding the culture medium, PTC uses AdDMEM/F12 as the basal medium, supplemented with N-acetyl-L-cysteine, fibroblast growth factor-7/10 (FGF-7/10), SB202190 (a p38 Mitogen-Activated Protein Kinase [MAPK] inhibitor), and other relevant components; salivary gland cancer uses complete medium (with Y-27632 supplemented during passage); HNSCC uses Advanced DMEM as the basal medium, containing B27 cell supplement, FGF-b, prostaglandin E2 (PGE2), and Forskolin. This evidence concerns the gene FGF7 and head and neck squamous cell carcinoma.