On the basis of reference marker genes (epithelial/cancer cell: EPCAM; immune cell: PTPRC, CD3G, CD3E, CD79A; stromal cell: BGN, PECAM1), the 22 clusters were broadly categorized into three major cell types: epithelial/cancer cells (clusters 2, 3, 6, 10, 12, 18), immune cells (clusters 0, 1, 4, 5, 7, 8, 9, 15, 16, 17, 19, 20), and stromal cells (clusters 11, 13, 14, 21) (Figure 11B). The gene discussed is PECAM1; the disease is cancer.