Upon analysis of all NK cells on a CD8/CD161 dot plot (Figure 2A) across three studied groups, we identified a significant increase in the frequency of CD8+CD161+ NK cells in T1D group compared to HD (p < 0.0001) and to T2D group (p = 0.0012) (Figure 2B, Table 3). This evidence concerns the gene KLRB1 and type 1 diabetes mellitus.