The validation datasets included MB subtypes from GSE203174 and CBTTC datasets (Figures 5a,b), H3 wildtype and mutant high grade gliomas (HGG), and diffuse midline gliomas (DMG) (Figures 5c,d), GN and NB from GSE147635 (Figure 5e), and GD2-high/low parental Kelly cells (NB) stained with GD2-APC antibody from GSE180514 (Figure 5f). This evidence concerns the gene APC and diffuse midline glioma.