To this end, we transfected plasmids expressing EGFP-α-synuclein, EGFP-parkin, EGFP-Tau, or EGFP-TDP43 (as a proxy for each neurodegenerative disease) into control or CLPB-KO HEK293T cells, or co-transfected them along with the CLPB expression vector. Here, MAPT is linked to neurodegenerative disease.