To this end, we transfected plasmids expressing EGFP-α-synuclein, EGFP-parkin, EGFP-Tau, or EGFP-TDP43 (as a proxy for each neurodegenerative disease) into control or CLPB-KO HEK293T cells, or co-transfected them along with the CLPB expression vector. The gene discussed is SNCA; the disease is neurodegenerative disease.