To elucidate the functional role of YTHDF1 in stemness, we utilized three-dimensional CSC cultures and CRC patient-derived organoids (PDOs) to recapitulate tumor physiology.20,21 In CSC28 and LS174TS cells, YTHDF1 overexpression enhanced self-renewal capacity (Fig. 1a), spheroid formation (Fig. 1b), and cell viability (Supplementary Fig. 1a). This evidence concerns the gene YTHDF1 and colorectal carcinoma.