Finally, we evaluated YTHDF1 mRNA expression in our in-house CRC patient cohort (cohort II, N = 151), revealing that YTHDF1 was positively correlated with two established colorectal CSC markers, CD133 (r = 0.23, P = 0.0043) and LGR5 (r = 0.33, P < 0.0001) (Fig. 8a), in tumor tissues, whereas the housekeeping gene ACTB was not correlated with either gene (Supplementary Fig. 5a). This evidence concerns the gene LGR5 and colorectal carcinoma.