Due to the cytotoxic action of the isolated L-asparaginase from Streptomyces rochei and Bacillus velezensis against Hep-G2 cells, several researchers had confirmed the efficiency of this enzyme on liver cancer Hep-G2 cells [90, 91] and also reported high IC50 4.0 IU from Streptomyces fradiae NEAE-82 [76]. The gene discussed is ASPG; the disease is liver cancer.