The top-most significant pleiotropic associations were between lead variants increasing risk for both AS and risk of disorders of lipid metabolism, occurring in lead variants prioritized for PSRC1, PSCK9, LPA, TRIB1, ZPR1 and LDLR. Other notable pleiotropic associations for binary traits include the AS risk variant rs56094641 (FTO) and increased risk for both obesity and diabetes, and the AS risk variant rs780093 (GCKR) and increased risk of gout and cirrhosis. This evidence concerns the gene ZPR1 and diabetes mellitus.