These observations were further supported by the anchorage-dependent colony formation assay, where USP11-KO HCT-15 and USP11-KO HT-29 cells formed significantly fewer colonies than the control cells (Supplementary Fig. S7K, L, USP11-KO HCT-15 vs. Ctrl HCT-15; Supplementary Fig. S7M, N, USP11-KO HT-29 vs. Ctrl HT-29), indicating that USP11 expression influences the migration, proliferation, and colony-forming ability of CRC cells. The gene discussed is USP11; the disease is colorectal carcinoma.