Following two days of culture to stabilize spheroids, tumor spheroids were treated with 49 nM mitoxantrone in HCT-15 cells or 40 nM mitoxantrone in HT-29 cells, alongside vehicle, EGF, HKLM (for TLR2), or LPS (for TLR4), and then the size of spheroids were measured for 10 days in HCT-15 and for 12 days in HT-29 cells, as illustrated in Fig. 7E. The gene discussed is TLR2; the disease is neoplasm.