Increased regulatory T cells (Tregs) in IAC samples suggested an immunosuppressive trend during tumor progression (Fig. 3e), potentially regulated by cytokines such as interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNF-α) (Fig. 3c-g and S3c-f). This evidence concerns the gene TNF and neoplasm.