To investigate the essential impact of MYD88 on the activation of immune modulation and AA pathway induced by SARS-CoV-2-Spike protein infection, cells treated with MYD88-specific shRNA or MYD88 inhibitors (ST2825, 20 μM and Myd88-IN-1, 5 μM) were established in BEAS-2B cells transfected with SARS-CoV-2-Spike to evaluate the regulatory effects of ACE2-MYD88 signals on cellular disturbance of metabolism and immune activity. This evidence concerns the gene CHMP5 and infection.