HFD feeding for 12 weeks successfully induced fatty liver and liver injury in mice, which were manifested by significant increase in hepatic triglyceride, glycogen, plasma triglyceride concentration, glucose, total cholesterol, LDL-cholesterol, ALT, AST, GGT and bilirubin levels compared with those of normal diet-fed mice (Figures 3A–K). This evidence concerns the gene GPT and fatty liver disease.