We therefore developed a new macrophage-specific SPP1 TAM signature (PLAUR, SLC11A1, BRI3, FBP1, C15orf48) using publicly available scRNA-Seq data from lung, colorectal and breast cancers (14) and validated the signature using multiple independently published scRNA-Seq datasets (Supplementary Figure 5B). This evidence concerns the gene SPP1 and breast carcinoma.