The ROC curve analysis showed that the areas under the curve (AUC) for tRF-Gly-GCC-1-M3, tRF-Met-CAT-2-M4, and tRF-SeC-TCA-1 in diagnosing multiple myeloma (MM) were 0.9844, 0.9773, and 0.9557, respectively, demonstrating that tRF-Gly-GCC-1-M3 exhibited the highest diagnostic efficacy (Figures 7G–I). The gene discussed is GCC1; the disease is Miyoshi myopathy.