To further evaluate the additive diagnostic potential of these tsRNAs, we performed a combined ROC analysis integrating tRF-Gly-GCC-1-M3, tRF-Met-CAT-2-M4, and tRF-SeC-TCA-1 to assess their overall discriminative capacity in multiple myeloma (MM) diagnosis. The gene discussed is GCC1; the disease is plasma cell myeloma.