GPT and urolithiasis: In another study, AgNPs (∼30 nm average size, 10 mg/kg) synthesized using Bryophyllum pinnatum leaf extract were used to treat urolithiasis (i.e., kidney stones) with a substantial increase in serum total protein, albumin, and globulin and a significant decrease in AST, ALT, creatinine, BUN, calcium, and phosphorus, compared to the untreated group (i.e., with urolithiasis but not treated with AgNPs) [159].