The principal strength of this study lies in its pioneering application of multimodal AFM to investigate TNF-α mediated calcium dysregulation in breast cancer cells at unprecedented resolution.​​ By integrating four complementary AFM modalities, SMRI, SMFS, CAFM, and KPFM, the research achieves simultaneous nanoscale spatial mapping, picoNewton level force sensitivity, picoampere current precision, and millivolt surface potential accuracy. The gene discussed is TNF; the disease is breast carcinoma.