It also reduced serum interleukin-1 (49.39±2.40 vs 43.51±1.64 pg/mL, p = 0.0350), haptoglobin (58.29±3.3 vs 49.40±3.65 μg/mL, p = 0.0050), and cortisol (37.42±1.67 vs 33.05±2.24 nmol/L, p = 0.0310), while increasing immunoglobulin G (10.30±1.46 vs 13.50±1.58 μg/mL, p = 0.0131) and interferon-γ (6.89±0.47 vs 7.94±0.63 ng/L, p = 0.0330) on D5.<h4>Conclusion</h4>MBAS alleviates weaning stress by inhibiting inflammation, enhancing immunity, and reducing anxiety, providing an effective strategy to improve calf health. The gene discussed is HP; the disease is Anxiety.