We next examined the levels of glycosylated PD-L1 in ten pairs of HCC tissues and noncancerous liver tissues by western blotting analysis, and found that PD-L1 was highly expressed in most cancer tissues (7/10) and appeared as fully glycosylated proteins (45 kDa) compared with noncancerous liver tissues (Fig. 5b). This evidence concerns the gene CD274 and hepatocellular carcinoma.