Annexin V/PI flow cytometry showed that BC–DOX-Lipo achieved the highest total apoptosis (44.51 ± 4.45%) and deep G0/G1 arrest (92.06 ± 9.2%), reducing S-phase to 6.37 ± 0.60% (p = 0.0186 vs DOX-Lipo). This evidence concerns the gene ANXA5 and breast cancer.