ASCL1 and small cell lung carcinoma: Quantitative real-time PCR (qPCR) bulk analysis of common SCLC molecular subtype markers (ASCL1, NEUROD1, POU2F3, YAP1; SCLC-A/N/P/Y) within RP and RPC tumors showed no overall significant difference in marker expression and a predominant expression of ASCL1 in both genotypes (Fig. 1k).