qPCR analysis confirmed the differential expression of genes related to neuronal differentiation which were found in the top 50 “up” or “down” regulated genes, like Six3, Pax3, Dlk1 or Dcx, but also Calca and Sox1, which were identified within snRNA-seq of tumors and strongly relate to the neuronal identity of SCLC (Supplementary Fig. 5b). This evidence concerns the gene SIX3 and small cell lung carcinoma.