We comprehensively analyzed differential methylation, survival analysis (Kaplan-Meier), correlation (Spearman’s), and pathway enrichment analysis (GO/KEGG) of CG genes (SYCP1, ADAD1, SYCE1, PRSS54, DMRTC2, and TEX101) in BC and leukemia. The gene discussed is PRSS54; the disease is leukemia.