Concurrently, we employed seven machine learning methods, we initially screened out 315 hub genes, and then employed (SVM, LASSO, KNN, XGB, GBM, C5.0, NNET) to mine diagnostic genes differentiating APOE ε3/ε4 female healthy controls from APOE ε3/ε4 female Alzheimer’s disease patients. This evidence concerns the gene APOE and Alzheimer disease.