Transient overexpression of Myc-MSL1 in LNCaP (prostate cancer), HCT116 (colon cancer), MCF-7 (breast cancer), and HeLa (cervical cancer) cells consistently elevated CD274 protein levels, as shown by Western blotting (Figure 3H, quantification in Figure 3I). The gene discussed is MSL1; the disease is Familial prostate cancer.