Since CAFs, the predominant stromal component in HCC, originate from activated fibroblasts/myofibroblasts during chronic liver injury [24] and critically orchestrate tumor progression through extracellular matrix (ECM) remodeling and paracrine signaling [33], we performed subtyping annotation of fibroblasts to delineate CAFs heterogeneity. This evidence concerns the gene TBX1 and hepatocellular carcinoma.