This decrease aligns with the IHC staining showing that intratumoral neutrophils enriched for SPP1 and VEGFA—markers of terminally differentiated, pro-angiogenic neutrophils—were abundant in KPC tumors but markedly reduced in KPC;Ulk1fl/fl tumors, consistent with their established role in promoting tumor vascularization and growth43,44. This evidence concerns the gene SPP1 and neoplasm.