However, enhanced pan-AKT (Thr308 and Ser473) phosphorylation was observed in double PIPP/PTEN knockdown cells relative to single PI-phosphatase knockdown or controls (Supplementary Fig. S5C, D), indicating that loss of both PI-phosphatases enhances AKT activation in breast cancer cell lines. The gene discussed is PTEN; the disease is breast carcinoma.