To validate this regulatory axis, we examined the expression of SRF and its coactivator, MRTF.33 Analysis of the human AD dataset (GSE63063) revealed that SRF (SRF) and MKL1 (MRTF-A) were significantly upregulated in the brains of patients with mild cognitive impairment (MCI) and AD, whereas MKL2 (MRTF-B) was not (Supplementary Fig. 8d). The gene discussed is SRF; the disease is Cognitive impairment.