We further analyzed the survival curve using the gene expression profiling interactive analysis (GEPIA)38 and Kaplan–Meier Plotter39.We found that low expression of NEO1(Fig. 1b) and UNC5D(Fig. 1d) showed poor prognosis in BLCA, whereas the high expression of UNC5B showed poor prognosis (Fig. 1c). This evidence concerns the gene NEO1 and bladder transitional cell carcinoma.