LAMP1 and melanoma: It has been reported that granzyme B typically localizes to lysosome-related organelles (LROs) in immune cells, which are positive for LAMP1 staining.53 In A375 melanoma cells, the colocalization of granzyme B with LAMP1 has been observed.47 Similarly, under control conditions, we found that granzyme B localized to LAMP1-positive foci in A375 cells, whereas DdBIC treatment not only induced granzyme B expression (Supplementary Fig. 6e) but also caused its diffuse distribution throughout the cytoplasm, resulting in partial colocalization with GSDMC in the cytoplasm (Supplementary Fig. 6j).