We found that DdBIC treatment-induced pyroptosis in the tumor model was closely associated with the activation of CD8+ T cells and NK cells, which is consistent with the established concept that pyroptosis functions as an immunogenic cell death mechanism capable of activating antitumor immunity.8 However, it remains unclear whether DdBIC can induce durable immune memory, a question that warrants further investigation through tumor rechallenge studies in future work. This evidence concerns the gene CD8A and neoplasm.