It has been reported that granzyme B typically localizes to lysosome-related organelles (LROs) in immune cells, which are positive for LAMP1 staining.53 In A375 melanoma cells, the colocalization of granzyme B with LAMP1 has been observed.47 Similarly, under control conditions, we found that granzyme B localized to LAMP1-positive foci in A375 cells, whereas DdBIC treatment not only induced granzyme B expression (Supplementary Fig. 6e) but also caused its diffuse distribution throughout the cytoplasm, resulting in partial colocalization with GSDMC in the cytoplasm (Supplementary Fig. 6j). This evidence concerns the gene GZMB and melanoma.