Second, DdBIC-induced pyroptotic morphologies were not reversible in melanoma A375 cells treated with other types of cell death, including Z-VAD (apoptosis), NSA (necroptosis), Nec-1 (an RIPK1 inhibitor that indirectly inhibits necroptosis), Fer-1 and Lip-1 (ferroptosis), CQ (autophagy), and TTM (cuproptosis) (Supplementary Fig. 1c), indicating that DdBIC is specific for pyroptotic induction. Here, PCSK1 is linked to melanoma.