CD44 and neoplasm: ,55,56 As expected, following a 72-h incubation with PαCD3&LIGHT-transfected tumor cells, the mRNA levels of CD69, CD44, and CD25 (activation markers) were upregulated, while those of PD-1, TIM-3, LAG-3, and CD39 (exhaustion markers) were downregulated, indicating effective activation and reduced exhaustion of OT-1 cells (Figure 4N).