A telomerase reverse transcriptase (TERT)-dependent expression plasmid is constructed and encapsulated into (2,3-dioleoyloxy-propyl)trimethylammonium chloride (DOTAP), polyethylene glycol (PEG), and poly lactic-co-glycolic acid (PLGA) nanoparticles (DOTAP-PEG-PLGA NPs) to fabricate the tumor-specific genetic plasmid vector (PαCD3&LIGHT). Here, TERT is linked to neoplasm.