Specifically, the DEPs involved in endosome transport via multivesicular body sorting pathway, neurotrophin receptor activity, and Golgi cisterna membrane mainly included Sort1, Vps28, Chsy3, and Golim4, which were linked with the initiation of autophagy and all downregulated in the hippocampus of Tg4510 mice exposed to sevoflurane, suggesting the involvement of autophagy in sevoflurane‐induced cognitive impairment. This evidence concerns the gene GOLIM4 and Cognitive impairment.