To furtherexplore the anticancer mechanism of PLK2 inhibition, a BrdU incorporationassay was conducted to assess its effect on cancer cell proliferation.The results demonstrated that 8012-3246 markedly inhibited DNA synthesisin a dose-dependent manner, indicating an antiproliferative effect.The GI50 values for growth inhibition were determined tobe 3.28 μM for HT-29 and 6.62 μM for HCT-116 (Figure C). Here, PLK2 is linked to cancer.