We also measured SLFN5 expression upon infection with HIV-1-N74D or HIV-1-A77V, which express a capsid protein that does not interact with CPSF6, and found that these mutant viruses failed to induce SLFN5 expression (Fig. 4D), suggesting that this induction is dependent on the interaction between the capsid protein and CPSF6. This evidence concerns the gene SLFN5 and infection.