To obtain additional data on the connection between capsid-CPSF6 interaction and increased SLFN5 expression, we challenged A549 cells with HIV-1-GFP at MOI=2 for 48 h in the presence or not of reverse transcriptase (RT) inhibitor Nevirapine (Nev), which doesn’t prevent CPSF5 and CPSF6 translocation to nuclear speckles during infection. This evidence concerns the gene SLFN5 and infection.