To obtain additional data on the connection between capsid-CPSF6 interaction and increased SLFN5 expression, we challenged A549 cells with HIV-1-GFP at MOI=2 for 48 h in the presence or not of reverse transcriptase (RT) inhibitor Nevirapine (Nev), which doesn’t prevent CPSF5 and CPSF6 translocation to nuclear speckles during infection. Here, NUDT21 is linked to infection.