PLK1 and cancer: Following that, a wide range of computational tools like SIFT, PolyPhen-2, E-SNPs and GO, MutPred2, FATHMM-XF, I-Mutant 2.0, CUPSAT, DynaMut2, mCSM, and so on were utilized for prioritizing high-risk nsSNPs of PLK1 in all types of cancers (Navapour and Mogharrab, 2021; Yadav and Singh, 2021; Kamal et al., 2024).