The method reported a LOD of 2.15 × 106 particles/μL using MUC1 probe for gastric cancer cell line HGC-27-derived EV sample, as well as validation for other gastric cancer biomarkers (EpCAM, PTK7, PD-L1) for both cell line EV isolates and human plasma specimens. The gene discussed is MUC1; the disease is gastric cancer.