In our study, 2 patients with AC with known adrenal adenoma were initially investigated for possible ACTH-dependent CS with IVDST, based on falsely elevated ACTH levels (performed on the Immulite platform) which were subsequently found to be due to assay interference, as shown by low ACTH levels of <2.2 pmol/L on a second assay platform; 1 of these 2 patients had other laboratory tests to confirm ACTH assay interference. Here, POMC is linked to adrenal cortex adenoma.