These in silico findings were substantiated by in vivo experimental validation in an ovalbumin-induced murine asthma model, demonstrating dose-dependent anti-inflammatory effects of Fructus Xanthii aqueous extract, including reduced cytokine levels (TNF-α, IL-6, IL-1β, IL-5), ameliorated lung histopathology, and downregulated expression of hub genes (HSP90AB1, CCNB1, CASP9, PI3K, AKT1). Here, TNF is linked to asthma.