Fibrosis, caspase-3 expression, and accumulations of tumor necrosis factor-α (TNF-α; P < 0.05) and interleukin-1β (IL-1β; P < 0.05), along with an elevated macrophage composition (P < 0.05) characterized the orchitis under HS. This evidence concerns the gene IL1B and histiocytic sarcoma.