Next, we investigated the clinical significance of TPX2-associated genes, including RRM2, CENPA, MYBL2, EXO1 and NEIL3, which were identified by WGCNA and top ranked through ML analysis, by assessing their prognostic significance in relation to Gleason score, tumor stage, and PSA levels obtained from clinical data from TCGA-PRAD13. This evidence concerns the gene KLK3 and neoplasm.