Multiple viruses such as CSFV, HIV-1, HCV, pseudorabies virus, HPV-16, Rhinovirus etc. utilize the actin machinery to facilitate their transport within the cell [56–61], A study from our laboratory performed an RNA interference-based screening of 136 human membrane trafficking genes in IMR-32 cells (human neuroblastoma) and identified RHOA, RAC1, PAK1, along with ARP2/3 complex, and N-WASP family proteins to be essential for JEV replication [17]. The gene discussed is RAC1; the disease is neuroblastoma.