In this study, CaP NPs stabilizedwith carboxymethyl cellulose (CMC) and coated with a silica layerwere designed for gemcitabine (GEM) loading and folate (FA) conjugation,targeting cancer cells overexpressing folate receptor alpha (FRα).GEM was covalently coupled to CMC via an amide bond before CaP precipitation,creating a prodrug system. This evidence concerns the gene FOLR1 and cancer.