Our results demonstrated that under WSSV infection, knockdown of Src64B or PI3Kp85α, or treatment of shrimp with the Src inhibitor Saracatinib or the PI3K inhibitor LY294002, significantly reduced PIP3 generation and Akt phosphorylation in hemocytes post-WSSV infection (Fig. S2A through H). Here, AKT1 is linked to infection.