SMAD4 and colon adenocarcinoma: To determine if FO chemotherapy-induced intestinal injury affects liver metastasis burden independently of its direct cytotoxic effect and to mimic the adjuvant treatment, we intrasplenically injected mice with either Apc-/-; KrasLSLG12D; Tp53-/-;Smad4-/--mCherry (AKPS) organoids or MC38-GFP mouse colon adenocarcinoma cells five days after FO treatment (Fig. 1g).